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ILNAS-EN ISO 21286:2020 Edition 04/2020
Soil quality - Identification of ecotoxicological test species by DNA barcoding (ISO 21286:2019)
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Abstract

This document specifies a protocol to identify ecotoxicological test specimens (mainly invertebrates and plants) to the species level, based on the DNA barcoding technique. This protocol can be used by laboratories performing DNA barcoding in order to standardize both the wet-lab and data analysis workflows as much as possible, and make them compliant with community standards and guidelines. This document does not intend to specify one particular strain for each test method, but to accurately document the species/strain which was used. NOTE 1 This does not imply that DNA barcoding is performed in parallel to each test run, but rather regularly (e.g. once a year, such as reference substance testing) and each time a new culture is started or new individuals are added to an ongoing culture. This document does not aim at duplicating or replacing morphological-based species identifications. On the contrary, DNA barcoding is proposed as a complementary identification tool where morphology is inconclusive, or to diagnose cryptic species, in order to ensure that the results obtained from different ecotoxicological laboratories are referring to the same species or strain. This document is applicable to identifications of immature forms which lack morphological diagnostic characters (eggs, larvae, juveniles), as well as the streamline identification of specimens collected in field monitoring studies, where large numbers of organisms from diverse taxa are classified. NOTE 2 In principle, all species regularly used in ecotoxicological testing can be analysed by DNA barcoding. Besides the earthwoms Eisenia fetida and E. andrei, further examples for terrestrial species are Lumbricus terrestris, L. rubellus, Allolobophora chlorotica, Aporrectodea rosea, and A. caliginosa, Dendrodrilus rubidus, Enchytraeus albidus, and E. crypticus (Haplotaxida); Folsomia candida, F. fimetaria, Proisotoma minuta, and Sinella curviseta (Collembola); Hypoaspis aculeifer and Oppia nitens (Acari); Aleochara bilineata and Poecilus cupreus (Coleoptera); Scathophaga stercoraria, Musca autumnalis (Diptera) or Pardosa sp. (Arachnida). Nematodes or snails and even plants can also be added to this list.

Status

Standard - Active

Origin

Technical Committee :
CEN/TC 444 : Test methods for environmental characterization of solid matrices

Implementation

start of the vote on the project    19/12/2019   date of ratification (dor)    13/04/2020
end of the vote on the project    12/03/2020   date of anouncement (doa)    31/07/2020
start of the vote on the final project    18/12/2020   date of publication (dop)    31/10/2020
end of the vote on the final project    12/02/2021   date of withdrawal (dow)    31/10/2020


Publication Official Journal
of the Grand-Duchy of Luxembourg 		21/10/2020
Reference Mémorial A N° 855

International Classification for Standards (ICS codes) :

13.080.30 : Biological properties of soils
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