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prEN ISO 19040-1 Edition 09/2023
Water quality - Determination of the estrogenic potential of water and waste water - Part 1: Yeast estrogen screen (Saccharomyces cerevisiae) (ISO 19040-1:2018)
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This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water; — waste water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.


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Technical Committee :
CEN/TC 230 : Water analysis


start of the vote on the project    26/05/2022   date of ratification (dor)    10/07/2023
end of the vote on the project    18/08/2022   date of anouncement (doa)    11/12/2023
start of the vote on the final project    13/04/2023   date of publication (dop)    11/03/2024
end of the vote on the final project    08/06/2023   date of withdrawal (dow)    11/03/2024

Publication Official Journal
of the Grand-Duchy of Luxembourg

International Classification for Standards (ICS codes) :

13.060.70 : Examination of biological properties of water


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