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FprEN 17899 Edition 07/2024
Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality
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Abstract

This document describes a spectrophotometric method for determining the chlorophyll-a content corrected for phaeopigments as a measure of the amount of phytoplankton for all types of surface water including marine water. The determination limit is usually 2 µg/l to 5 µg/l and is calculated by each laboratory individually. It can be as low as 0,5 µg/l using 2 l of sample (or even more) and a 50 mm cuvette. NOTE In some measurement programs like marine studies on time series data and ecological status/classification no correction for phaeopigments is used and acidification is omitted, e.g. as recommended by OSPAR.

Status

Final draft - Active

Origin

Technical Committee :
CEN/TC 230 : Water analysis

Implementation

start of the vote on the project    22/09/2022   date of ratification (dor)    06/06/2024
end of the vote on the project    15/12/2022   date of anouncement (doa)    04/10/2024
start of the vote on the final project    14/03/2024   date of publication (dop)    06/01/2025
end of the vote on the final project    09/05/2024   date of withdrawal (dow)    06/01/2025


Publication Official Journal
of the Grand-Duchy of Luxembourg
Reference

International Classification for Standards (ICS codes) :

13.060.70 : Examination of biological properties of water
71.040.50 : Physicochemical methods of analysis

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