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prEN 14105 Edition 10/2024
Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of free and total glycerol and mono-, di-, triglyceride contents
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This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results. Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks. NOTE 1 For the purposes of this document, the term "% (m/m)" is used to represent the mass fraction. NOTE 2 Under the common EN 14105 GC conditions, squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate. WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.


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Technical Committee :
CEN/TC 307 : Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis


start of the vote on the project    09/03/2023   date of ratification (dor)    13/09/2024
end of the vote on the project    01/06/2023   date of anouncement (doa)    13/01/2025
start of the vote on the final project    21/06/2024   date of publication (dop)    11/04/2025
end of the vote on the final project    16/08/2024   date of withdrawal (dow)    11/04/2025

Publication Official Journal
of the Grand-Duchy of Luxembourg


Relations to older standards
ILNAS-EN 14105:2020

International Classification for Standards (ICS codes) :

67.200.10 : Animal and vegetable fats and oils


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