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ILNAS-EN 17547:2021 Edition 11/2021
Animal feeding stuffs: Methods of sampling and analysis - Determination of vitamin A, E and D content - Method using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC)
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Abstract

This document specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (α-tocopherol) and vitamin D3 (cholecalciferol) in animal feed using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC). NOTE The procedure also enables determination of vitamin D2 but with the use of another internal standard. The method is fully validated only for vitamin D3. The method has been successfully tested in collaborative trial for complete feed for broilers, pigs, and turkey, for premixture for broilers and piglets, for complementary feed for cows and mineral feed within the following ranges: • vitamin A: 4 365 IU/kg - 4 118 352 IU/kg; • vitamin E: 22 mg/kg - 13 800 mg/kg; • vitamin D3: 1 668 IU/kg - 1 638 150 IU/kg. The limits of quantification were not determined within the validation study. Quantification limits of 1 100 IU for vitamin A/kg (using UV-detection), 4 mg for vitamin E/kg (using UV-detection), 2 mg for vitamin E/kg (using fluorescence detection) and 2 000 IU for vitamin D/kg (using UV-detection) should be normally achieved. Lower limits are possible provided they are validated by the user.

Status

Standard - Active

Origin

Technical Committee :
CEN/TC 327 : Animal feeding stuffs - Methods of sampling and analysis

Implementation

start of the vote on the project    13/08/2020   date of ratification (dor)    27/09/2021
end of the vote on the project    05/11/2020   date of anouncement (doa)    28/02/2022
start of the vote on the final project    01/07/2021   date of publication (dop)    31/05/2022
end of the vote on the final project    26/08/2021   date of withdrawal (dow)    31/05/2022


Publication Official Journal
of the Grand-Duchy of Luxembourg 		24/05/2022
Reference Mémorial A N° 244

International Classification for Standards (ICS codes) :

65.120 : Animal feeding stuffs
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